Article
A novel approach for the identification of protein-protein interaction with integral membrane proteins.
Nucleic acids research - 15 Feb 2001
Hubsman M, Yudkovsky G, Aronheim A
Abstract excerpt
Protein-protein interaction plays a major role in all biological processes. The currently available genetic methods such as the two-hybrid system and the protein recruitment system are relatively limited in their ability to identify interactions with integral membrane proteins. Here we describe the development of a reverse Ras recruitment system (reverse RRS), in which the bait used encodes a membrane protein....
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