Article
Tracking a protein following dissociation from a protein-protein complex using a split SNAP-tag system.
Analytical biochemistry - 15 May 2015
Mie Masayasu, Naoki Tatsuhiko, Kobatake Eiry
Abstract excerpt
Protein-protein interactions (PPIs) are important for various biological processes in living cells. Several methods have been developed for the visualization of PPIs in vivo; however, these methods are unsuitable for visualization of post-PPI events such as dissociation and translocation. In this study, we applied a split SNAP-tag system for the visualization of post-PPI events. This method enabled tracking of...
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