Article
Adaptation of the Ras-recruitment system to the analysis of interactions between membrane-associated proteins.
Nucleic acids research - 15 Mar 2003
Köhler Fabian, Müller Kristian M
Abstract excerpt
Interactions of membrane-associated proteins play important roles in many cellular processes. The yeast two-hybrid assay is of limited utility for the analysis of such interactions, due to the need for soluble protein partners, whose interaction is assessed in the nucleus. The advent of the Ras-recruitment system (RRS) has enabled the study of membrane-associated proteins interacting with cytoplasmic proteins...
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