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Article

Direct sequencing from touch preparations of human carcinomas: analysis of p53 mutations in breast carcinomas.

Journal of the National Cancer Institute - 17 Jul 1991

Kovach J S, McGovern R M, Cassady J D, Swanson S K, Wold L E, Vogelstein B, Sommer S S

Abstract excerpt

A new technique for characterizing somatic mutations in very small samples of cellularly heterogeneous human cancer tissue was developed and tested using mutations in the p53 gene in breast carcinomas as a model system. The technique combines touch preparation of specimens to obtain homogeneous c...

Topics

Adenocarcinoma
Base Sequence
Breast Neoplasms
Eosine Yellowish-(YS)
Female
Genes, p53
Hematoxylin
Humans
Methylene Blue
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tolonium Chloride

Tumor Cells, Cultured

Source

Identifiers

PMID: 1712853
DOI: 10.1093/jnci/83.14.1004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1712853-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Direct sequencing from touch preparations of human carcinomas: analysis of p53 mutations in breast carcinomas.PMID 1712853Read ↗

Analysis of p53 mutations in single cells obtained from histological tissue sections.Semantic proximity Read ↗

Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma.Semantic proximity Read ↗

Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation.Semantic proximity Read ↗

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Pattern of p53 gene mutations in breast cancers of women of the midwestern United States.Semantic proximity Read ↗

DNA sequence analysis of exons 2 through 11 and immunohistochemical staining are required to detect all known p53 alterations in human malignancies.Semantic proximity Read ↗

Molecular and immunohistochemical analysis of p53 mutations in scrapings and tissue from preinvasive and invasive breast cancer.Semantic proximity Read ↗

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