Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Direct sequencing from touch preparations of human carcinomas: analysis of p53 mutations in breast carcinomas.

Journal of the National Cancer Institute - 17 Jul 1991

Kovach J S, McGovern R M, Cassady J D, Swanson S K, Wold L E, Vogelstein B, Sommer S S

Abstract excerpt

A new technique for characterizing somatic mutations in very small samples of cellularly heterogeneous human cancer tissue was developed and tested using mutations in the p53 gene in breast carcinomas as a model system. The technique combines touch preparation of specimens to obtain homogeneous clusters of carcinoma cells free of normal cells with a nested pair of polymerase chain reaction (PCR) amplifications of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenocarcinoma
Share to Base Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Breast Neoplasms

Share to Genes, p53

Share to Molecular Sequence Data

Share to Mutation

Share to Polymerase Chain Reaction

Share to Tumor Cells, Cultured

Source

Identifiers

PMID: 1712853
DOI: 10.1093/jnci/83.14.1004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Direct sequencing from touch preparations of human carcinomas: analysis of p53 mutations in breast carcinomas.PMID 1712853Read ↗

Analysis of p53 mutations in single cells obtained from histological tissue sections.Semantic proximity Read ↗

Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma.Semantic proximity Read ↗

Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation.Semantic proximity Read ↗

p53 Mutation analysis in breast tumors by a DNA microarray method.Semantic proximity Read ↗

Pattern of p53 gene mutations in breast cancers of women of the midwestern United States.Semantic proximity Read ↗

DNA sequence analysis of exons 2 through 11 and immunohistochemical staining are required to detect all known p53 alterations in human malignancies.Semantic proximity Read ↗

Molecular and immunohistochemical analysis of p53 mutations in scrapings and tissue from preinvasive and invasive breast cancer.Semantic proximity Read ↗

Detection of single base alterations in genomic DNA by solid phase polymerase chain reaction on oligonucleotide microarrays.Semantic proximity Read ↗

APEX disease gene resequencing: mutations in exon 7 of the p53 tumor suppressor gene.Semantic proximity Read ↗

Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.Semantic proximity Read ↗

Rapid p53 sequence analysis in primary lung cancer using an oligonucleotide probe array.Semantic proximity Read ↗

Screening of the entire coding region of p53 in low grade lymphoproliferative disorders.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.