Article
Detection of single base alterations in genomic DNA by solid phase polymerase chain reaction on oligonucleotide microarrays.
Analytical biochemistry - 1 Dec 2001
Huber M, Losert D, Hiller R, Harwanegg C, Mueller M W, Schmidt W M
Abstract excerpt
DNA microarray technology holds significant promise for human DNA diagnostics. A number of technical approaches directed at the parallel identification of mutations or single nucleotide polymorphisms make use of polymerase-based specificity, like minisequencing or allele-specific primer elongation. These techniques, however, require separate laborious sample amplification, preparation, and purification steps,...
Topics
- Base Composition
- Colonic Neoplasms
- DNA, Neoplasm
- Genes, p53
- Genome
- Humans
- Mutation, Missense
- Oligonucleotide Array Sequence Analysis
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide