Article
Molecular beacon-based real-time PCR method for detection of 15 high-risk and 5 low-risk HPV types.
Journal of virological methods - 1 Apr 2008
Takács Tibor, Jeney Csaba, Kovács Laura, Mózes Johanna, Benczik Márta, Sebe Attila
Abstract excerpt
Detection of HPV infections requires a robust time-effective single-step method for efficient screening. A molecular beacon-based one-step multiplex real-time PCR system was developed to detect 15 high-risk (HPV types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) and 5 low-risk HPV types (HPV types 6, 11, 42, 43, 44). Molecular beacons detecting high-risk types are 5'-FAM-3'-DABCYL-labelled,...
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