Article
Quantification of two variant strains contained in freeze-dried Japanese BCG vaccine preparation by real-time PCR.
Biologicals : journal of the International Association of Biological Standardization - 1 Apr 2007
Shibayama Keigo, Mochida Keiko, Yagi Tetsuya, Mori Shigetaro, Arakawa Yoshichika, Yamamoto Saburo
Abstract excerpt
Japanese bacillus Calmette-Guerin (BCG) vaccine preparation contains two types of variant strains, Type I, which has a 22-base-pair deletion in the RD16 region, and Type II, which has an identical sequence to those of other BCG strains. In this study, we established a method to quantify the percentage of variant strain Type II contained in freeze-dried BCG product with real-time PCR. With this method we examined...
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