Article
Multiplex-PCR as an identity assay for Mycobacterium bovis BCG Moreau descendants.
Biologicals : journal of the International Association of Biological Standardization - 1 May 2013
Krysztopa-Grzybowska Katarzyna, Brzezińska Sylwia, Augustynowicz-Kopeć Ewa, Polak Maciej, Lutyńska Anna
Abstract excerpt
In the study, we assessed the identity of locally produced BCG vaccine via screening for the presence of genetic markers specific for particular Mycobacterium bovis BCG substrains - RD8, RD2, senX3-regX3, RD14, RD16, ΔRD1, DU2, a second copy of IS6110, mutation D322G in phoR, and deletions in fadD26-ppsA and Rv3887c regions. In order to increase the specificity of the multiplex-PCR test for locally produced BCG...
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