Article
Identification of two subpopulations of Bacillus Calmette-Guérin (BCG) Tokyo172 substrain with different RD16 regions.
Vaccine - 5 Jun 2006
Honda Ikuro, Seki Masaaki, Ikeda Noriko, Yamamoto Saburo, Yano Ikuya, Koyama Akira, Toida Ichiro
Abstract excerpt
Two types of colonies with different morphologies (smooth: S and rough: R) formed when Bacillus Calmette-Guérin (BCG) Tokyo172 substrain was cultured on Middlebrook 7H10 agar medium, and their genotypes were analyzed by multiplex PCR on five RD regions and SenX3-RegX3. In most cases these two colony types had different genotypes, i.e., S colonies showed a characteristic 22 bp deletion in Rv3405c of the RD16...
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