Article
Proline-rich motifs in the parathyroid hormone (PTH)/PTH-related protein receptor C terminus mediate scaffolding of c-Src with beta-arrestin2 for ERK1/2 activation.
The Journal of biological chemistry - 15 Dec 2006
Rey Alexandre, Manen Danielle, Rizzoli René, Caverzasio Joseph, Ferrari Serge L
Abstract excerpt
Parathyroid hormone (PTH) stimulates ERK1/2 through both G-protein signaling and beta-arrestin2-mediated internalization. Beta-arrestin may serve as a scaffold for c-Src. However, the molecular mechanisms for ERK1/2 activation by PTH remain unclear. By using a targeted mutagenesis approach, we investigated the PTH/PTH-related protein receptor (PTH1R) structural determinants for ERK1/2 activation and...
Topics
- Amino Acid Sequence
- Arrestins
- Cell Line
- Cyclic AMP
- Enzyme Activation
- Humans
- MAP Kinase Signaling System
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Molecular Sequence Data
- Mutation
- Peptides
- Proline-Rich Protein Domains