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Article

A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGK.

Journal of molecular biology - 8 Dec 2006

Cliff Matthew J, Alizadeh Tooba, Jelinska Clare, Craven C Jeremy, Staniforth Rosemary A, Waltho Jonathan P

Abstract excerpt

Protein folding is directed by the sequence of sidechains along the polypeptide backbone, but despite this the developement of sidechain interactions during folding is not well understood. Here, the thiol-active reagent, dithio-nitrobenzoic acid (DTNB), is used to probe the exposure of the cysteine sidechain thiols in the kinetic folding intermediates of the N-terminal domain of phosphoglycerate kinase (N-PGK)...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17030040
DOI: 10.1016/j.jmb.2006.09.014

Genes

DTNB

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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