Characterization of an intermediate in the folding pathway of phosphoglycerate kinase: chemical reactivity of genetically introduced cysteinyl residues during the folding process.

doi:10.1021/bi00053a040

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Article

Characterization of an intermediate in the folding pathway of phosphoglycerate kinase: chemical reactivity of genetically introduced cysteinyl residues during the folding process.

Biochemistry - 19 Jan 1993

Ballery N, Desmadril M, Minard P, Yon J M

Abstract excerpt

The unfolding-refolding kinetics of yeast phosphoglycerate kinase were studied using the chemical reactivity of genetically introduced cysteinyl residues as conformational probes and far-ultraviolet circular dichroism. A unique internal cysteinyl residue was introduced in several mutants at selec...

Topics

Circular Dichroism
Cysteine
Kinetics
Mutation
Phosphoglycerate Kinase
Protein Folding
Saccharomyces cerevisiae
Sulfhydryl Compounds

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