Article
A general strategy for site-specific double labeling of globular proteins for kinetic FRET studies.
Bioconjugate chemistry - 1 Jan 2000
Ratner V, Kahana E, Eichler M, Haas E
Abstract excerpt
Site-directed mutagenesis provides a straightforward means of creating specific targets for chemical modifications of proteins. This capability enhanced the applications of spectroscopic methods adapted for addressing specific structural questions such as the characterization of partially folded and transient intermediate structures of globular proteins. Some applications such as the steady state or time-resolved...
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