Article
A comparison of direct infusion MS and GC-MS for metabolic footprinting of yeast mutants.
Biotechnology and bioengineering - 1 Apr 2007
Mas Sandrine, Villas-Bôas Silas Granato, Hansen Michael Edberg, Akesson Mats, Nielsen Jens
Abstract excerpt
Recent technical advances in mass spectrometry (MS) have propelled this technology to the forefront of methods employed in metabolome analysis. Here, we compare two distinct analytical approaches based on MS for their potential in revealing specific metabolic footprints of yeast single-deletion mutants. Filtered fermentation broth samples were analyzed by GC-MS and direct infusion ESI-MS. The potential of both...
Topics
- Fermentation
- Gas Chromatography-Mass Spectrometry
- Genomics
- Mass Spectrometry
- Mutation
- Proteomics
- Saccharomyces cerevisiae