Article
High-throughput profiling of amino acids in strains of the Saccharomyces cerevisiae deletion collection.
Genome research - 1 Sept 2010
Cooper Sara J, Finney Gregory L, Brown Shauna L, Nelson Sven K, Hesselberth Jay, MacCoss Michael J, Fields Stanley
Abstract excerpt
The measurement of small molecule metabolites on a large scale offers the opportunity for a more complete understanding of cellular metabolism. We developed a high-throughput method to quantify primary amine-containing metabolites in the yeast Saccharomyces cerevisiae by the use of capillary electrophoresis in combination with fluorescent derivatization of cell extracts. We measured amino acid levels in the yeast...
Topics
- Amino Acids
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genetic Variation
- Metabolomics
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins