Article
High-throughput quantitative metabolomics: workflow for cultivation, quenching, and analysis of yeast in a multiwell format.
Analytical chemistry - 1 May 2009
Ewald Jennifer Christina, Heux Stéphanie, Zamboni Nicola
Abstract excerpt
Metabolomics is a founding pillar of quantitative biology and a valuable tool for studying metabolism and its regulation. Here we present a workflow for metabolomics in microplate format which affords high-throughput and yet quantitative monitoring of primary metabolism in microorganisms and in particular yeast. First, the most critical step of rapid sampling was adapted to a multiplex format by using fritted...
Topics
- Chromatography, Gas
- Metabolome
- Metabolomics
- Microbiological Techniques
- Mutation
- Reproducibility of Results
- Saccharomyces cerevisiae