SP6 RNA polymerase stutters when initiating from an AAA... sequence.

doi:10.1093/nar/19.17.4669

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Article

SP6 RNA polymerase stutters when initiating from an AAA... sequence.

Nucleic acids research - 11 Sept 1991

Cunningham P R, Weitzmann C J, Ofengand J

Abstract excerpt

The 16S ribosomal RNA gene of Escherichia coli was placed under the transcriptional control of consensus and modified T7 promoters and a modified SP6 promoter. Both T7 and SP6 polymerases faithfully transcribed the coding sequence (beginning at the +1 position) of each construct, although SP6 pol...

Topics

Bacteriophages
Base Sequence
DNA, Ribosomal
DNA-Directed RNA Polymerases
Escherichia coli
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Promoter Regions, Genetic
RNA, Ribosomal, 16S
Substrate Specificity
Transcription, Genetic
Viral Proteins

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