Article
Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers.
PLoS genetics - 29 Sept 2006
Geurts Aron M, Collier Lara S, Geurts Jennifer L, Oseth Leann L, Bell Matthew L, Mu David, Lucito Robert, Godbout Susan A, Green Laura E, Lowe Scott W, Hirsch Betsy A, Leinwand Leslie A, Largaespada David A
Abstract excerpt
Previous studies of the Sleeping Beauty (SB) transposon system, as an insertional mutagen in the germline of mice, have used reverse genetic approaches. These studies have led to its proposed use for regional saturation mutagenesis by taking a forward-genetic approach. Thus, we used the SB system to mutate a region of mouse Chromosome 11 in a forward-genetic screen for recessive lethal and viable phenotypes. This...
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