Article
Random mutagenesis of proximal mouse chromosome 5 uncovers predominantly embryonic lethal mutations.
Genome research - 1 Aug 2005
Wilson Lawriston, Ching Yung-Hao, Farias Michael, Hartford Suzanne A, Howell Gareth, Shao Hongguang, Bucan Maja, Schimenti John C
Abstract excerpt
A region-specific ENU mutagenesis screen was conducted to elucidate the functional content of proximal mouse Chr 5. We used the visibly marked, recessive, lethal inversion Rump White (Rw) as a balancer in a three-generation breeding scheme to identify recessive mutations within the approximately 50 megabases spanned by Rw. A total of 1003 pedigrees were produced, representing the largest inversion screen...
Topics
- Animals
- Behavior, Animal
- Chromosomes
- Embryonic Development
- Ethylnitrosourea
- Female
- Fertility
- Genes, Lethal
- Genetic Complementation Test
- Gestational Age
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Inbred Strains