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Article

Ca2+ selectivity of a chemically modified OmpF with reduced pore volume.

Biophysical journal - 15 Dec 2006

Miedema Henk, Vrouenraets Maarten, Wierenga Jenny, Gillespie Dirk, Eisenberg Bob, Meijberg Wim, Nonner Wolfgang

Abstract excerpt

We studied an E. coli OmpF mutant (LECE) containing both an EEEE-like locus, typical of Ca(2+) channels, and an accessible and reactive cysteine. After chemical modification with the cysteine-specific, negatively charged (-1e) reagents MTSES or glutathione, this LECE mutant was tested for Ca(2+) versus alkali metal selectivity. Selectivity was measured by conductance and zero-current potential. Conductance...

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Identifiers

PMID: 16997866
DOI: 10.1529/biophysj.106.087114
PMCID: PMC1779923

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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