Article
Nucleosome rearrangement in human cells following short patch repair of DNA damaged by bleomycin.
Biochemistry - 14 Aug 1990
Sidik K, Smerdon M J
Abstract excerpt
We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesis was resistant to aphidicolin. In both intact and permeabilized cells, nascent repair patches...
Topics
- Alkylating Agents
- Bleomycin
- Cell Membrane Permeability
- Cells, Cultured
- Chromatin
- DNA
- DNA Damage
- DNA Polymerase I
- DNA Repair
- DNA Replication
- Humans
- Mutation