Article
Brassica oleracea.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Sparrow Penny A C, Dale Philip J, Irwin Judith A
Abstract excerpt
A better understanding of the genetic basis underlying the genotype dependence of Brassica oleracea transformation is enabling researchers to distinguish between recalcitrant and successful candidate genotypes for routine transformation. In this chapter we outline an A. tumefaciens-mediated transformation method for B. oleracea using 4-d-old cotyledonary explants and a model B. oleracea doubled haploid genotype,...
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