Article
Medicago truncatula transformation using cotyledon explants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Wright Elane, Dixon Richard A, Wang Zeng-Yu
Abstract excerpt
Medicago truncatula has been developed into a model species for legumes. Large numbers of ESTs have been sequenced, and sequencing of the complete gene-rich space of the genotype Jemalong A17 is in progress. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be...
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