Article
Choice of Explant for Plant Genetic Transformation.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Chakraborty Nibedita, Chakraborty Priyanka, Sen Moutushi, Bandopadhyay Rajib
Abstract excerpt
Particle bombardment or biolistic transformation is an efficient, versatile method. This method does not need any vector for the gene transfer and is not dependent on the cell type, species, and genotype. The success of any transformation technique depends on the starting experimental materials or the explants. Here, we describe the factors that have influenced the choice of explants in biolistic transformation....
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