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Article

Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures.

BMC plant biology - 19 May 2011

Ribas Alessandra F, Dechamp Eveline, Champion Anthony, Bertrand Benoît, Combes Marie-Christine, Verdeil Jean-Luc, Lapeyre Fabienne, Lashermes Philippe, Etienne Hervé

Abstract excerpt

BACKGROUND: Following genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or...

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PMID: 21595964
DOI: 10.1186/1471-2229-11-92
PMCID: PMC3111370

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