Article
High throughput Agrobacterium tumefaciens-mediated germline transformation of mechanically isolated meristem explants of cotton (Gossypium hirsutum L.).
Plant cell reports - 1 Jan 2014
Chen Yurong, Rivlin Anatoly, Lange Andrea, Ye Xudong, Vaghchhipawala Zarir, Eisinger Elizabeth, Dersch Erik, Paris Miriam, Martinell Brian, Wan Yuechun
Abstract excerpt
KEY MESSAGE: Agrobacterium tumefaciens mediates high frequency of germline transformation of cotton meristem explants. The meristem transformation system we developed is rapid, high throughput and genotype-flexible. We have developed a high throughput cotton transformation system based on direct Agrobacterium inoculation of mechanically isolated meristem explants of cotton (Gossypium hirsutum L.). The explants...
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