Article
Peas (Pisum sativum L.).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Grant Jan, Cooper Pauline
Abstract excerpt
In this chapter we describe a robust method for transformation of peas that has been successfully used in our laboratory since 1992. Using immature pea seed collected from field- or greenhouse-grown plants, we have produced transgenic lines for over 30 genotypes including named pea cultivars and advanced breeding lines. This method uses immature cotyledons as the explant, and the transformation efficiency is in...
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