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Article

Alfalfa (Medicago sativa L.).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006

Samac Deborah A, Austin-Phillips Sandra

Abstract excerpt

A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest. The explants and bacteria are cocultured for 7 to 8 d. Bacteria are removed by rinsing explants...

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Source

Identifiers

PMID: 16988354
DOI: 10.1385/1-59745-130-4:301

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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