Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.

Folia microbiologica - 1 Jan 1990

Bachvarov D, Jay E, Ivanov I

Abstract excerpt

In order to construct plasmids bearing inducible high-copy-number phenotype, the cloning plasmid pBR322 was modified as follows: a DNA fragment containing a strong synthetic promoter (P1), synthetic lac operator (O1), DNA sequence corresponding to the RNAI/RNAII region of the Co1E1 replicon and t...

Topics

Bacteriocin Plasmids
Cloning, Molecular
DNA, Bacterial
Gene Expression Regulation, Bacterial
Lac Operon
Phenotype
Promoter Regions, Genetic
RNA, Bacterial
Replicon
Terminator Regions, Genetic
Transformation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 1698694
DOI: 10.1007/BF02820482

Genes

CAT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1698694-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.PMID 1698694Read ↗

High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacB by Introducing inc Mutations.Semantic proximity Read ↗

Improvement of pCOR plasmid copy number for pharmaceutical applications.Semantic proximity Read ↗

Yeast expression vectors using RNA polymerase III promoters.Semantic proximity Read ↗

The Bacteriophage Lambda CII Phenotypes for Complementation, Cellular Toxicity and Replication Inhibition Are Suppressed in cII-oop Constructs Expressing the Small RNA OOP.Semantic proximity Read ↗

Identification of a high-copy-number plasmid suppressor of a lethal phenotype caused by mutant DnaA protein which has decreased intrinsic ATPase activity.Semantic proximity Read ↗

Repressible promoters - a novel tool to generate conditional mutants in Pichia pastoris.Semantic proximity Read ↗

Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria.Semantic proximity Read ↗

High-frequency gene replacement in cyanobacteria using a heterologous rps12 gene.Semantic proximity Read ↗

Phenotypic variation caused by variation in the relative copy number of pDU1-based plasmids expressing the GAF domain of Pkn41 or Pkn42 in Anabaena sp. PCC 7120.Semantic proximity Read ↗

An expression system for regulated protein production in Synechocystis sp. PCC 6803 and its application for construction of a conditional knockout of the ferrochelatase enzyme.Semantic proximity Read ↗

Mutations within the replicon of the IncN plasmid pCU1 that affect its Escherichia coli polA-independence but not its autonomous replication ability.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.