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Article

Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.

Folia microbiologica - 1 Jan 1990

Bachvarov D, Jay E, Ivanov I

Abstract excerpt

In order to construct plasmids bearing inducible high-copy-number phenotype, the cloning plasmid pBR322 was modified as follows: a DNA fragment containing a strong synthetic promoter (P1), synthetic lac operator (O1), DNA sequence corresponding to the RNAI/RNAII region of the Co1E1 replicon and the CAT gene transcription terminator was substituted for the 29 bp EcoRI/HindIII DNA fragment. Two types of plasmids...

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Share to Cloning, Molecular
Share to DNA, Bacterial
Share to CAT gene

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Bacterial

Share to Lac Operon

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Share to Promoter Regions, Genetic

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Source

Identifiers

PMID: 1698694
DOI: 10.1007/BF02820482

Genes

CAT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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