Article
Repressible promoters - a novel tool to generate conditional mutants in Pichia pastoris.
Microbial cell factories - 24 Jan 2013
Delic Marizela, Mattanovich Diethard, Gasser Brigitte
Abstract excerpt
BACKGROUND: Repressible promoters are a useful tool for down-regulating the expression of genes, especially those that affect cell viability, in order to study cell physiology. They are also popular in biotechnological processes, like heterologous protein production. RESULTS: Here we present five novel repressible Pichia pastoris promoters of different strength: PSER1, PMET3, PTHR1, PPIS1 and PTHI11. eGFP was...
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