Article
An expression system for regulated protein production in Synechocystis sp. PCC 6803 and its application for construction of a conditional knockout of the ferrochelatase enzyme.
Journal of biotechnology - 30 Nov 2012
Kuchmina E, Wallner T, Kryazhov S, Zinchenko V V, Wilde A
Abstract excerpt
Synechocystis sp. PCC 6803 is a model organism for the study of photosynthetic processes. Methods to genetically manipulate this bacterium are essential to investigate these processes and to evaluate potential biotechnological applications. We developed a vector for controllable expression of proteins using a platform for stable integration of the expression cassette into the genome. The respective gene is...
Topics
- Biotechnology
- Cloning, Molecular
- Copper
- Ferrochelatase
- Gene Knockout Techniques
- Genetic Vectors
- Phenotype
- Phycocyanin
- Plasmids
- Promoter Regions, Genetic
- Recombinant Proteins