High-frequency gene replacement in cyanobacteria using a heterologous rps12 gene.

doi:10.1093/pcp/pch041

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Article

High-frequency gene replacement in cyanobacteria using a heterologous rps12 gene.

Plant & cell physiology - 1 Mar 2004

Takahama Kazutaka, Matsuoka Masayoshi, Nagahama Kazuhiro, Ogawa Takahira

Abstract excerpt

Multiple targeted gene replacements are often required for functional analyses of cyanobacterial genomes. For this purpose, we previously devised a simple genetic method, termed rps12-mediated gene replacement, in a cyanobacterium Synechococcus elongatus PCC 7942 for construction of mutants free from drug resistance markers. Here, we improved the method by employing a heterologous rps12 gene encoding a ribosomal...

Topics

Anti-Bacterial Agents
Base Sequence
Cyanobacteria
Drug Resistance, Bacterial
Gene Deletion
Genes, Bacterial
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Phenotype
Plasmids
Recombination, Genetic
Ribosomal Proteins
Species Specificity
Streptomycin
Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 15047882
DOI: 10.1093/pcp/pch041

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