Article
Kinase activity of mutant LRRK2 mediates neuronal toxicity.
Nature neuroscience - 1 Oct 2006
Smith Wanli W, Pei Zhong, Jiang Haibing, Dawson Valina L, Dawson Ted M, Ross Christopher A
Abstract excerpt
Mutations in the the leucine-rich repeat kinase-2 (LRRK2) gene cause autosomal-dominant Parkinson disease and some cases of sporadic Parkinson disease. Here we found that LRRK2 kinase activity was regulated by GTP via the intrinsic GTPase Roc domain, and alterations of LRRK2 protein that reduced kinase activity of mutant LRRK2 correspondingly reduced neuronal toxicity. These data elucidate the pathogenesis of...
Topics
- Analysis of Variance
- Blotting, Western
- Cell Survival
- Cells, Cultured
- Cerebral Cortex
- Green Fluorescent Proteins
- Guanosine Triphosphate
- Humans
- Immunohistochemistry
- In Situ Nick-End Labeling
- Leucine-Rich Repeat Serine-Threonine Protein Kinase-2