Article
Advantage of using inosine at the 3' termini of 16S rRNA gene universal primers for the study of microbial diversity.
Applied and environmental microbiology - 1 Nov 2006
Ben-Dov Eitan, Shapiro Orr H, Siboni Nachshon, Kushmaro Ariel
Abstract excerpt
To overcome the shortcomings of universal 16S rRNA gene primers 8F and 907R when studying the diversity of complex microbial communities, the 3' termini of both primers were replaced with inosine. A comparison of the clone libraries derived using both primer sets showed seven bacterial phyla amplified by the altered primer set (8F-I/907R-I) whereas the original set amplified sequences belonging almost exclusively...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.