Article
454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature.
BioMed research international - 1 Jan 2015
Gonzalez-Martinez Alejandro, Rodriguez-Sanchez Alejandro, Rodelas Belén, Abbas Ben A, Martinez-Toledo Maria Victoria, van Loosdrecht Mark C M, Osorio F, Gonzalez-Lopez Jesus
Abstract excerpt
Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for the Bacteria domain able to amplify the anammox 16S rRNA genes. A metagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in five autotrophic...
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