Article
Biased diversity metrics revealed by bacterial 16S pyrotags derived from different primer sets.
PloS one - 1 Jan 2013
Cai Lin, Ye Lin, Tong Amy Hin Yan, Lok Si, Zhang Tong
Abstract excerpt
In recent years, PCR-based pyrosequencing of 16S rRNA genes has continuously increased our understanding of complex microbial communities in various environments of the Earth. However, there is always concern on the potential biases of diversity determination using different 16S rRNA gene primer...
Topics
- Bacteria
- Cluster Analysis
- DNA Primers
- Genetic Variation
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA
- Temperature