Article
16 S rDNA primers and the unbiased assessment of thermophile diversity.
Biochemical Society transactions - 1 Apr 2004
Baker G C, Cowan D A
Abstract excerpt
Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. "Universal" and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to the degree of specificity of primer pairs to di...
Topics
- Base Sequence
- DNA
- DNA Primers
- DNA, Ribosomal
- Genes, Archaeal
- Genetic Variation
- Genome, Archaeal
- Hot Temperature
- Methanococcus
- Molecular Sequence Data
- Nucleic Acid Conformation
- Nucleotides
- Phylogeny
- Polymerase Chain Reaction
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Pyrococcus
- RNA, Ribosomal