Can composition and structural features of oligonucleotides contribute to their wide-scale applicability as random PCR primers in mapping bacterial genome diversity?

doi:10.1016/s0167-7012(02)00040-4

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Article

Can composition and structural features of oligonucleotides contribute to their wide-scale applicability as random PCR primers in mapping bacterial genome diversity?

Journal of microbiological methods - 1 Aug 2002

Limansky Adriana S, Viale Alejandro M

Abstract excerpt

Among current genotypic methodologies, random amplification of polymorphic DNA (RAPD or AP-PCR) represents a widely employed assay for the evaluation of bacterial genomic diversity. A common bottleneck of this technique, however, is represented by the screening of useful informative primers to di...

Topics

5' Flanking Region
Bacteria
DNA Primers
Genetic Variation
Genome, Bacterial
Random Amplified Polymorphic DNA Technique
Reproducibility of Results

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