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Article

The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies.

PloS one - 1 Jan 2013

Ghyselinck Jonas, Pfeiffer Stefan, Heylen Kim, Sessitsch Angela, De Vos Paul

Abstract excerpt

Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced. We wanted to gain insight in the impact of primer choice on the outcome of short read sequencing efforts. All the unknowns associated with sequencing data, i.e. primer coverage rate, phylogeny, OTU-richness and taxonomic...

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Identifiers

PMID: 23977026
DOI: 10.1371/journal.pone.0071360
PMCID: PMC3747265

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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