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Article

Complementary retrieval of 16S rRNA gene sequences using broad-range primers with inosine at the 3'-terminus: implications for the study of microbial diversity.

FEMS microbiology ecology - 1 Jan 2010

Brands Berit, Vianna Morgana E, Seyfarth Ilse, Conrads Georg, Horz Hans-Peter

Abstract excerpt

We evaluated the impact of the base analogue inosine substituted at the 3'-terminus of broad-range 16S rRNA gene primers on the recovery of microbial diversity using terminal restriction fragment length polymorphism and clonal analysis. Oral plaque biofilms from 10 individuals were tested with modified and unmodified primer pairs. Besides a core overlap of shared terminal restriction fragments (T-RFs), each...

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PMID: 19807788
DOI: 10.1111/j.1574-6941.2009.00786.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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