Identification of amino acid residues within simian virus 40 capsid proteins Vp1, Vp2, and Vp3 that are required for their interaction and for viral infection.

Abstract excerpt

Interaction of simian virus 40 (SV40) major capsid protein Vp1 with the minor capsid proteins Vp2 and Vp3 is an integral aspect of the SV40 architecture. Two Vp3 sequence elements mediate Vp1 pentamer binding in vitro, Vp3 residues 155 to 190, or D1, and Vp3 residues 222 to 234, or D2. Of the two, D1 but not D2 was necessary and sufficient to direct the interaction with Vp1 in vivo. Rational mutagenesis of Vp3...

Topics

• Animals
• Capsid Proteins
• Cell Line
• Chlorocebus aethiops
• Humans
• Mutation
• Polyomavirus Infections
• Protein Conformation
• Protein Structure, Tertiary
• Recombinant Proteins
• Tumor Virus Infections