Article
The simian virus 40 minor structural protein Vp3, but not Vp2, is essential for infectious virion formation.
The Journal of general virology - 1 Aug 2003
Gharakhanian Editte, Muñoz Luz, Mayorca Luz
Abstract excerpt
The SV40 capsid is composed of pentameric capsomeres of the major structural protein Vp1. The two minor structural proteins, Vp2 and Vp3, interact with the capsid. Here, the roles of Vp2 and Vp3 were explored during the course of SV40 infection. Start codons of Vp2, Vp3, or both Vp2 and Vp3, were destroyed by site-directed mutagenesis, and mutant genomes were transfected into CV-1 cells. SV40DeltaVp2 produced...
Topics
- Animals
- Capsid
- Capsid Proteins
- Cell Line
- Mutagenesis, Site-Directed
- Mutation
- Simian virus 40
- Viral Plaque Assay
- Virion
- Virus Assembly