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Article

A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination.

BMC genomics - 4 Apr 2006

Missirlis Perseus I, Smailus Duane E, Holt Robert A

Abstract excerpt

BACKGROUND: Cre-loxP recombination refers to the process of site-specific recombination mediated by two loxP sequences and the Cre recombinase protein. Transgenic experiments exploit integrative recombination, where a donor plasmid carrying a loxP site and DNA of interest integrate into a recipient loxP site in a target genome. Unfortunately, integrative recombination is highly inefficient because the insert is...

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Identifiers

PMID: 16595017
DOI: 10.1186/1471-2164-7-73
PMCID: PMC1479339

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