Spontaneous and X-ray-induced deletion mutations in a LacZ plasmid-based transgenic mouse model.

Abstract excerpt

Transgenic mouse mutation models carrying bacterial marker genes in bacteriophage lambda shuttle vectors have been applied to study spontaneous or induced mutations in vivo. However, due to the nature of the shuttle vector these models are insensitive to large deletions. Clastogenic agents, which predominantly induce large deletions, were therefore found to yield very low responses in these assays. Here we report...

Topics

• Animals
• Gene Deletion
• Lac Operon
• Mice
• Mice, Transgenic
• Mutation
• Plasmids
• X-Rays