Article
The use of transgenic mice for short-term, in vivo mutagenicity testing.
Genetic analysis, techniques and applications - 1 Dec 1990
Kohler S W, Provost G S, Kretz P L, Fieck A, Sorge J A, Short J M
Abstract excerpt
In order to develop a short-term, in vivo assay to study the mutagenic effects of chemical exposure, transgenic mice were generated using a lambda shuttle vector containing a lacZ target gene. Following exposure to mutagens, this target can be rescued efficiently from genomic DNA prepared from tissues of the treated mice using restriction minus, in vitro lambda phage packaging extract and restriction minus...
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