The use of transgenic mice for short-term, in vivo mutagenicity testing.

doi:10.1016/0735-0651(90)90003-x

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Article

The use of transgenic mice for short-term, in vivo mutagenicity testing.

Genetic analysis, techniques and applications - 1 Dec 1990

Kohler S W, Provost G S, Kretz P L, Fieck A, Sorge J A, Short J M

Abstract excerpt

In order to develop a short-term, in vivo assay to study the mutagenic effects of chemical exposure, transgenic mice were generated using a lambda shuttle vector containing a lacZ target gene. Following exposure to mutagens, this target can be rescued efficiently from genomic DNA prepared from ti...

Topics

Animals
Bacteriophage lambda
Escherichia coli
Ethylnitrosourea
Genetic Vectors
Methylation
Mice
Mice, Transgenic
Mutagenicity Tests
Mutagens
Phenotype
Time Factors
beta-Galactosidase

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