Article
Molecular and genetic characterization of an ornithine decarboxylase-deficient Chinese hamster cell line.
The Journal of biological chemistry - 25 May 1990
Pilz R B, Steglich C, Scheffler I E
Abstract excerpt
The ornithine decarboxylase (ODC)-deficient Chinese hamster ovary (CHO) cell line C55.7 has normal amounts of ODC mRNA with very low amounts of immunologically detectable ODC protein, suggesting a structural mutation; however, 5-azacytidine treatment leads to phenotypical reversion (Steglich, C., and Scheffler, I. E. (1985) Somat. Cell Mol. Genet. 11, 11-23). We have demonstrated by chemical cleavage a single...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cell Line
- Cricetinae
- Cricetulus
- DNA
- Female
- Methylation
- Molecular Sequence Data
- Mutation