Article
Effect of mutations at active site residues on the activity of ornithine decarboxylase and its inhibition by active site-directed irreversible inhibitors.
The Journal of biological chemistry - 25 Nov 1993
Coleman C S, Stanley B A, Pegg A E
Abstract excerpt
Mouse ornithine decarboxylase (ODC) and mutants changing residues thought to be involved at the active site were expressed in Escherichia coli, purified to homogeneity by affinity chromatography on a pyridoxamine 5'-phosphate-agarose affinity column, and tested for their kinetic properties and th...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cell Line, Transformed
- DNA Primers
- Eflornithine
- Escherichia coli
- Mice
- Molecular Sequence Data
- Mutation
- Ornithine Decarboxylase
- Ornithine Decarboxylase Inhibitors
- Oxidation-Reduction
- Pyridoxal Phosphate
- Spectrum Analysis
- Sulfhydryl Compounds