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Article

Genetic selection for critical residues in ribonucleases.

Journal of molecular biology - 22 Sept 2006

Smith Bryan D, Raines Ronald T

Abstract excerpt

Homologous mammalian proteins were subjected to an exhaustive search for residues that are critical to their structure/function. Error-prone polymerase chain reactions were used to generate random mutations in the genes of bovine pancreatic ribonuclease (RNase A) and human angiogenin, and a genetic selection based on the intrinsic cytotoxicity of ribonucleolytic activity was used to isolate inactive variants....

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Identifiers

PMID: 16920150
DOI: 10.1016/j.jmb.2006.07.020

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Genetic selection for critical residues in ribonucleases.PMID 16920150Read ↗

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