New Method for the Quantitative Determination of Major Protein Carbonyls, α-Aminoadipic and γ-Glutamic Semialdehydes:  Investigation of the Formation Mechanism and Chemical Nature in Vitro and in Vivo

Abstract excerpt

Alpha-aminoadipic semialdehyde (AAS) and gamma-glutamic semialdehyde (GGS) are identified as the major carbonyl products in oxidized proteins. To elucidate the formation pathway of AAS and GGS in vivo, we developed and validated a new quantification method. AAS and GGS in proteins were derivatized by reductive amination with NaCNBH(3) and p-aminobenzoic acid, a fluorescent reagent, followed by acid hydrolysis. It...

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