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Article

The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: use of site-specific mutagenesis and recombinant protein expression.

The Journal of cell biology - 1 Apr 1990

Hatzfeld M, Weber K

Abstract excerpt

Recombinant DNA technology has been used to analyze the first step in keratin intermediate filament (IF) assembly; i.e., the formation of the double stranded coiled coil. Keratins 8 and 18, lacking cysteine, were subjected to site specific in vitro mutagenesis to change one amino acid in the same relative position of the alpha-helical rod domain of both keratins to a cysteine. The mutations lie at position -36 of...

Topics

Actin Cytoskeleton
Amino Acid Sequence
Animals
Cysteine
DNA, Recombinant
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression
Keratins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Macromolecular Substances

Mice

Microscopy, Electron

Molecular Sequence Data

Molecular Weight

Mutation

Protein Conformation

Recombinant Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 1691189
DOI: 10.1083/jcb.110.4.1199
PMCID: PMC2116092

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1691189-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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